*Some of the antimicrobial agents are coated according the CLSI，Document M43-A6, Methods for Antimicrobial Susceptibility Testing for Human Mycoplasmas.

There is Studies shown that UU is associated with NGU, male sperm quality, female pelvic inflammatory, but UP is considered as normal flora in these patients.3 UP can cause choroiditis, fetal systemic inflammatory response, premature delivery and fetal lung infection.4

The key to the treatment and prevention of the spread of Mycoplasma is timely and accurate diagnosis. Mycoplasma cultivation is currently still being recognized as a reliable method to diagnose the Mycoplasma infection.5

Note: The species UP (Ureaplasma parvum) and UU (Ureaplasma urealyticum) are considered together as Ureaplasma spp.

Mycoplasma TIES includes a selective medium and 20 test strips. The mixed medium is prepared by mixing the lyophilized powder and the diluent. After the sample with mixed medium has been cultured, urea within the culture broth can be decomposed by urease in Ureaplasma spp. and release NH3; arginine within the culture broth can be decomposed by arginase in MH and release NH3. NH3 increases the pH of the culture broth; the result is read according to the color change of the indicator. The species UP (Ureaplasma parvum) and UU (Ureaplasma urealyticum) are distinguished according to their different tolerance to manganese ions. The strip contains 12antimicrobialagents. If Mycoplasma is susceptible to antimicrobial agents, the activity of enzyme would be inhibited; hence there is no change in color.

1.Strip

2.Lyophilized Powder

Contains inhibition agent. The growth of interfering organisms could be inhibited while the growth of Mycoplasma could be promoted.

3.Mineral Oil

4.Diluent

used to dissolve the lyophilized powder.